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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was identified to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant part in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants were being described but in depth nucleotide sequence information and facts isn't nevertheless accessible.
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Blocking peptides are peptides that bind specifically on the focus on antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters website to ensure the very best results are...
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This guideline outlines even more possible optimization of cellular and immunoassay parameters to guarantee the best possible benefits are...
Antibodies sure to the blocking peptide no longer bind for the epitope to the concentrate on protein. By evaluating the staining in the blocked antibody compared to the antibody by yourself, one can see which staining is precise.
Concentrate on Information Cyclin E belongs to the extremely conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which contribute into the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is particularly degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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